Micropropagation of phalaenopsis orchids via protocorms and protocormlike bodies. Production of healthy plant materials by shoot tip meristem culture is a scientific and technical movie, illustrating the different stages of the meristem culture, that allows the regeneration. Protoplast isolation and culture, fusion of protoplast. Multiple shoot induction from axillary bud cultures of the. Find powerpoint presentations and slides using the power of, find free presentations research about micropropagation ppt. The growing of plant cells, tissues, organs, seeds or other plant parts in a sterile environment on a nutrient medium. Mar 25, 2017 plant tissue culture is an abbreviation for all invitro techniques for culturing, propagating and manipulating plant cell,tissue or organ cultures in an aseptic environment on proper growth medium. In vitro shoot induction and plant regeneration from. Photoautotrophic micropropagation using sugarfree medium is an area of potential utility to scaleup potato micropropagation for commercialization, and recent advancements in the development and utilization of large culture vessels and case studies concerning photoautotrophic micropropagation were described by zobayed et al. Batra department of vegetable science, ccshau, hisar125001, haryana, india corresponding author a b s t r a c t value in basic research for the production of true to type, disease free and vitro plant tissue culture of potato solanum tuberosum l. The authors provide a comprehensive worldwide survey of. Micropropagation is the rapid vegetative propagation of plants under in vitro conditions of high light intensity, controlled temperature, and a defined nutrient medium. Thaiszia journal of botany, 2010 this title comes as two separate hardcover volumes, attractively housed together within a 5sided cardboard casing. It grows as a lithophyte or epiphyte and is characterized by short, stout pseudo bulbs with 2 to 4 leathery.
Find powerpoint presentations and slides using the power of, find free presentations research about micropropagation of banana ppt. Micropropagation is the art and science of plant multiplication in vitro. View and download powerpoint presentations on micropropagation ppt. One of singapores critically endangered species, robiquetia spathulata, has been successfully cloned and reintroduced using this method.
A detailed collection of procedures and methods for multiplying orchids, including organ, tissue, and cell culture techniques in vitro presents classic techniques. Orchid culture media plant tissue culture sigmaaldrich. Multiple shoot induction from axillary bud cultures of the medicinal orchid, dendrobium longicornu stadwelson dohling, 1 suman kumaria, 2, and pramod tandon 2 1 department of botany, lady keane college, shillong, meghalaya 793 001, india. As its name suggest tissue culture is done by using plant tissue, mostly the. Plant tissue culture micropropagation is growing smart grow. By using crosssectioned flower buds fbs, we found that in both. In vitro micropropagation of orchid vanda tessellata l. Introduction to tissue culture tissue culture also known as micropropagation or in vitro culture is. Plant tissue culture micropropagation is growing smart. Shoot multiplication of paphiopedilum orchid through in. Sometimes, when there is only one plant left in the natural habitat, the only way to bulk up the population is by micropropagation.
Toshio murashige university of california micropropagation. Key is to grasp concepts and extrapolate, use imagination. Dendrobium peguanum lindl is a delightful miniature from india, thailand and burma. There may be other alternate materials and approaches.
This is a compendium of methods for in vitro propagation of orchids. A guidebook in orchid micropropagation by marco acuna issuu. A guidebook in orchid micropropagation produced by. A detailed collection of procedures and methods for multiplying orchids, including organ, tissue, and cell culture techniques in vitro.
It originated as a solution for hard to germinate orchids, but has been sprouting this new standard throughout the cannabis community and shows great promise for high production farming. Micropropagation of orchids micropropagation is the process used to replicate plants such as orchids, the word micropropagation means simply propagation in miniature. Principles of tissue culture for micropropagation is the property of its rightful owner. Jan 15, 2020 micropropagation stages, types, applications, advantages, limitations. Biotechnology, specifically in vitro propagation, may be the only viable solution for preserving. Propagation is a means of keeping the plants healthy and produce new stock orchids can be propagated in several ways depending on their growth habit propagation a few ways vegetative, seed and tissue culture vegetative propagation is common, easy, popular and can be done with minimum facilities. Propagating orchids from tissue culture welcome to. Propagating orchids dividing plants is easier than using tiny orchid seeds, but propagating orchids is a challenge. Orchid culture media we offer two proven media formulations for the tissue culture of the orchid family.
Explants carrying performed vegetative bud suitable for enhanced axillary branching. Propagation a few ways vegetative, seed and tissue culture. Virus free plant sub millimeter portion of shoottip stock plant virus free nodal cutting disadvantage of using small sized explant for micropropagation shoot tip explant low survival rate and slow initial growth. Micropropagation is the property of its rightful owner. Propagating orchids from tissue culture welcome to propagation. Of the approximately 25,000 orchidaceae, many species are rare or endangered, others are prized horticulturally, and many are used in traditional medicine. Reliable rates and consistency of shoot multiplication. Ppt principles of tissue culture for micropropagation. When the plant grows large and overgrows its container. Thaiszia journal of botany, 2010 the book lives up to its billing as a useful resource strictly dedicated to micropropagation. Micropropagation of orchids by joseph arditti overdrive. Use of tissue culture technique for micro propagation was first started by morel 1960 for propagation of orchids, and is now applied to several plants. Ppt micropropagation powerpoint presentation free to view.
Orchids need repotting regularly, usually every two to three years. Today there is a wealth of knowledge about growing orchids and modern propagation methods have driven prices to affordable levels. It is done under sterile conditions in a laboratory using plant seed or tissue. Micropropagation is the practice of rapidly multiplying stock plant material to produce many progeny plants, using modern plant tissue culture methods. Jan 26, 2009 this greatly expanded and updated edition of a classic reference work comprises two volumes offering a compendium of methods for multiplying orchids through micropropagation. Historical figures in research on orchid seed germination and micropropagation, plant hormone studies and tissue culture research. View and download powerpoint presentations on micropropagation of banana ppt. The authors provide a comprehensive worldwide survey of the literature on the methods for multiplying orchid plants using tissue culture techniques. Micropropagation stages, types, applications, advantages. Micropropagation of paphiopedilum through tissue culture is a prerequisite for the commercialization of slipper orchid by the trade regulation of the convention on international trade in endangered species cites liao et al. In 1960, morel cultured shoot tips for obtaining virusfree cymbidium clones in vitro.
In vitro clonal propagation through tissue culture is referred to as micro propagation. Micropropagation of phalaenopsis orchids via protocorms and. Micropropagation of orchids, 2 volume set, 2nd edn article pdf available in annals of botany 1054 march 2010 with 1,430 reads how we measure reads. Though maximum reports on orchid micropropagation surveyed have used leaves as the starting material, popular use of leaf explantmediated. Pdf micropropagation of orchids, 2 volume set, 2nd edn. In vitro shoot induction and plant regeneration from flower. If so, share your ppt presentation slides online with. For each genus, there is information on culture and tables detailing the composition of. Advances in propagation techniques of orchids authorstream. An in vitro study on micropropagation of cymbidium orchids. Phalaenopsis orchids have high economic value in the floriculture industry. What distinguishes orchids from other flowering plants is the combination of three.
The first 2 chapters cover the history of orchid micropropagation and give a general outline of techniques and procedures. Members of the genus are sold and exhibited as pot plants and cut flowers. It is the fact that micropropagation is the only commercially viable method of clonal propagation of most of the horticultural crops. The secondary roots of the terrestlial orchid establi shed in the chopped forest litters during the process of hardening the regenerates gets. Because orchids are difficult to grow from seed, knudson c medium is used to support seed germination in tissue culture. Micropropagation is rapid, continuous, and efficient. Tissue culture techniques has helped orchids occupy a position as one of the top ten cut flowers.
We offer two proven media formulations for the tissue culture of the orchid family. A comprehensive guide to orchid culture venamy orchids. First commercially used with orchids conventional propagation rate of 1 per year. This study was aimed at inducing shoots and regenerating plants from the flowering plants of a sequentially flowering paphiopedilum deperle and a single floral paphiopedilum armeni white. The in vivo clonal propagation of plants is tedious, expensive and frequently unsuccessful.
Micropropagation can be used as means of such ex situ conservation. Wild populations of paphiopedilum are under the threat of extinction due to overcollection and loss of suitable habitats. Although tropical orchids can germinate on relatively simple media, the temperate orchids might require complex media or the presence of a symbiotic fungus. Today growing orchids is more than just a hobby, it is an international business covering around 8% of the world floriculture trade and has the potential to alter the economic landscape of a country. An in vitro study on micropropagation of cymbidium orchids natarajan kannan department of biotechnology, manipal institute of technology, manipal576104, karnataka, india email. Section d production of disease free plants by tissue culture methods. Micro propagation of orchids demonstrated profit all over the world, besides orchid about 600 species of other ornamental plants have been successfully cloned and some of them are commercially exploited chrysanthemum, carnation, gerbera and anthurium, list of names is increasing day by day, the advantage is rapid cloning of selected colour.
Thanks to dr shashidhar for sharing materials and advice. The percentage seed germination varied with the composition of the nutrient media and was highest in fullstrength ms basal medium. Here are a couple of quick facts that give an idea of the size of the orchid family. In 1949, rotor at cornell university demonstrated that plantlets could be induced by aseptic culturing of the dormant buds on the basal node of phalaenopsis inflorescence. Sep 10, 2014 aseptic method of clonal propagation is called as micropropagation and it offer the advantage of large number of truetotype plantlets can be produced with relatively short time and space from a single individual. My journey towards conservation the orchid society of.
Micropropagation of cymbidium sleeping nymph through. These are the newest techniques derived from current molecular biology research and offer great practical utility for growers of often rare and beautiful orchids. The technique has been applied to a substantial number of commercial vegetatively propagated plant species. Acclimatization of plants transferred to soil micropropagation successful plant transfer from culture to soil show high survival rate and cost involved in the process is low. To date, improved tissue culture methods using orchid roots, leaves, flower buds, stems and inflorescences have been adopted, making orchid. Production of healthy plant materials by shoot tip. An efficient protocol was established for in vitro propagation of c. It contains a great amount of information including the most up to date scientific results.
A free powerpoint ppt presentation displayed as a flash slide show on id. A detailed collection of procedures and methods for multiplying orchids, including. The numbers of plantlets offsprings obtainable are minimal 1. Micropropagation is the practice of rapidly multiplying stock plant material to produce many progeny plants, using modern plant tissue culture methods micropropagation is used to multiply plants such as those that have been genetically modified or bred through conventional plant breeding methods. In nature, many orchids are epiphytes, growing on other plants, and some are terrestrial, growing in the ground. Ppt micropropagation powerpoint presentation free to. Principles of tissue culture for micropropagation a free powerpoint ppt presentation displayed as a flash slide show on id.
Pdf in vitro micropropagation of orchid vanda tessellata l. These adventitious embryoids are diploid in nature and can be used as clonal material for micropropagation. This chapter focuses on two common micropropagation methods using seed and protocormlike body plb cultures, respectively. Common protocols in orchid micropropagation springerlink. Phytotechnology laboratories offers several media specifically developed for the in vitro culture of orchids. Paphiopedilum is one of the most popular and rare orchid genera.
An efficient in vitro hardening technique of tissue culture. Paphiopedilum species are recalcitrant in tissue culture, and no explant from mature plants has been successfully mass propagated in vitro. Transplanted plantlets shoot immediately irrigated with inorganic nutrient solution and maintained under high humidity for initial 1015 days. Recent advances in orchid tissue culture researchgate. Micropropagation stages, types, applications, advantages, limitations. Affordable prices, the fascination exercised by their captivating beauty and their diversity has made them increasingly popular houseplants. Despite a ban on the collection and trade of all orchid species in nepal, numerous anthropogenic factors are leading to the rapid loss of natural stands of germplasm. Transverse thin cell layers ttcls of protocormlike bodies of two stages of plbs 30 d and 60 d old of cymbidium sleeping nymph were used as explants to induce plbs by using coconut water cw as a natural additive.
The present investigation was undertaken to mass propagate cymbidium mastersii, an ornamental orchid of northeast india by in vitro propagation method. Presentation based on first hand experiences of 78 yrs of raising orchids by this method. Orchids can be propagated both sexually as well as asexually but due to numerous drawbacks in conventional method of propagation now a days various tissue culture techniques are being adopted for commercial production of orchids meristem culture is widely used technique. The process includes many stepsstock plant care, explant selection and sterilization, media manipulation to obtain proliferation, rooting, acclimation, and growing on of liners. With orchids it is the only way to reproduce the plants as. Sometimes, when there is only one plant left in the natural habitat, the only way to bulk up. Specialized equipment, facilities, and technically trained personnel are required. Plant tissue culture which has been around for decades, is a way to reproduce new plants from the mother tissue and is used as an alternative to cloning. This greatly expanded and updated edition of a classic reference work comprises two volumes offering a compendium of methods for multiplying orchids through micropropagation. A detailed collection of procedures and methods for multiplying orchids, including organ, tissue, and cell culture techniques in vitro presents classic techniques that have been in the forefront of orchid.
Orchids are members of the family of monocots known as orchidaceae. Though maximum reports on orchid micropropagation surveyed have used leaves as the starting material, popular use of leaf explant. Department of developmental and cell biology, university of california, irvine, ca926972300, u. Micro propagation is a handy technique for rapid multiplication of plants. With orchids it is the only way to reproduce the plants as naturally orchids are mutually beneficial with a type of fungi. Aseptic method of clonal propagation is called as micropropagation and it offer the advantage of large number of truetotype plantlets can be produced with relatively short time and space from a single individual. This is by some accounts the most diverse family of plants in the world, with over 25,000 species. Micropropagation of of orchis catasetum a rare and endangered orchid mohammad vali fakouri ghaziani1, ahmad baker1, naser negahdar2, behzad kaviani1 1islamic azad university, rasht branch, department of horticultural science, rasht, iran. Steps can be taken to obtain and maintain certified pestfree plants. Tech biotechnology, university school of biotechnology usbt, ggsip university, new delhi india. Orchids are prized for their beautiful long lasting flowers exhibiting an incredible range of diversity in size, shape and colour. How to keep orchids alive and looking gorgeous when propagating orchids from tiny seeds, special care must be taken. The roots of epiphytic orchids are achaing to the charcoal chips and i.